1.

論文

論文
Sato, Hideaki ; Sato, Masashi ; Chiba, Makie ; Ito, Kyoko ; Ito, Koichi
出版情報: 弘前医学.  61  pp.35-45,  2010-03-25.  弘前大学出版会
URL: http://hdl.handle.net/10129/3278
概要: The ability of murine allogeneic umbilical cord blood cells (UCBCs) to reconstitute the immune systemwas investigated. U CBCs obtained from fetuses of C57BL/6 (B6; H-2b) mice, which were transgenic for greenfluorescent protein (GFP), were transplanted into RAG2 (-/-) BALB/c mice (H-2d). After transplantation, flowcytometric analysis revealed successful reconstitution of phenotypically mature GFP-positive immune cells of donororigin, including T cells, B cells, monocytes, and granulocytes in the peripheral blood of the recipient mice. Analysisof functional maturation of lymphocytes revealed that 2,4,6-trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH)-immunized UCBC-transplanted recipients produced both TNP-specific IgM and IgG antibodies. These resultsindicated that the recipient mice were capable of mounting antibody responses to T-dependent antigens; further, Igclass switching from IgM to IgG confi rmed that both B cells and CD4+ helper T cells derived from allogeneic UCBCswere immunologically competent. Furthermore, mice transplanted with allogeneic UCBCs accepted skin grafts fromboth B6 and BALB/c mice. However, these chimeric mice completely rejected skin grafts from third party C3H/HeJ (H-2k) mice, indicating the presence of functional CD8+ killer T cells as well as CD4+ helper T cells. In termsof potential clinical application, our results indicate that allogeneic UCBC transplantation can enable recovery of thenormal immune system in recipients. 続きを見る
2.

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論文
Abe, Kazuhiro ; Higuchi, Tsuyoshi ; Mizunuma, Hideki ; Kakizaki, Ikuko ; Endo, Masahiko ; Suto, Shinichiro
出版情報: 弘前医学.  61  pp.97-103,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4346
概要: The aim of study is to analyze functional sugar chains, glycosaminoglycans (GAGs) in urine frompostmenopausal women with osteoporosis. A 24-hour urine was collected from 15 osteoporotic women and normalwomen. All women were postmenopausal and aged at 60 to 70 years old. Diagnosis of osteoporosis was based onthe diagnosis criteria proposed by the Japan Osteoporosis Society after measurement of lumbar spine bone mineraldensity by dual energy X-ray absorptiometry. The urine was concentrated by dialysis and evaporation, thenprecipitated with ethanol. The precipitate was then reconstituted into NaCl solution, precipitated again by adding 10% cetylpyridinium chloride and the precipitation was used as complex GAGs fraction. The yield of complex fractionwas significantly higher in osteoporotic subjects than that in normal control. Then, the complex GAGs fraction wasanalyzed by cellulose acetate membrane electrophoresis and high performance liquid chromatography (HPLC),respectively. Cellulose acetate membrane electrophoresis has revealed that GAGs in urine contained partiallydesulfated chondroitin sulfate. However, further analysis by HPLC has shown that the excretion of desulfated GAGswas rather less in osteoporotic women, indicating that urinary GAGs fraction from osteoporotic women containsunknown acidic polysaccharides as well as GAGs. 続きを見る
3.

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論文
大久保, 愉一 ; 松坂, 方士 ; 高橋, 一平 ; 檀上, 和真 ; 中路, 重之 ; 梅田, 孝
出版情報: 弘前医学.  61  pp.104-113,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4347
概要: 労働者のメンタルヘルスと社会環境学的要因を調査し,メンタルヘルスに及ぼすこれらの影響を調査した.東北,北海道の労働者6310名に家族構成,生活習慣,健康度,職場環境,抑うつ度(CES-D)をアンケートで聞き取り,CES-D と他の項目の関係 を検討した.その結果,「仕事・職業満足度」「仕事裁量度」「仕事士気度」「上司関係満足度」「自己評価点」「健康度」「生活習慣点」「家族・友人満足度」で,低得点群に比較し高得点群でオッズ比が小さく,「悪対処数(問題となるストレス解消行動の数)」ではその逆であった.一方,「勤務時間」「時間外勤務」「仕事量質負担度」では有意な結果は得られなかった.以上より,わが国の労働者は地域社会・家庭のストレスを職場に持ち込み,さらにうつに対する感受性がうつ度に大きく影響していることが示唆された.また,仕事量質の影響は大きくなく,労働者のメンタルヘルス改善を考えた場合,社会精神文化を考慮した職場環境を構築することが重要と考えられた. 続きを見る
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論文
川口, 英夫 ; 青木, 昌彦 ; 畑山, 佳臣 ; 小野, 修一
出版情報: 弘前医学.  61  pp.114-121,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4348
概要: 【目的】4D-CTを用いて呼吸性移動の線量計算に対する影響を検討した.【対象・方法】4D-CTを撮像した14例を対象に平均画像を用いて非対向6門で治療計画を作成した.計画を各呼吸位相のCTに貼り付けMU値を計算し,呼吸位相毎に平均画像のMU 値と比較した(MUdiff ).また,各門毎の変動を計算した(⊿MU).呼吸性移動をビームの平面方向と垂直方向に分離し,それぞれ⊿MUとの相関を検討した.線量計算はClarkson法(C法), superposition法(S法)の2つを用い比較した.【結果】MUdiff はC法では吸気相以外で,S法では終末吸気・呼気のみで有意差を認めた.⊿MUはC法では平面上方向・垂直方向に中等度相関を認めたが,S法では垂直方向のみに弱い相関を認めた.【結論】C法は呼吸性移動の影響を受けやすく,S法は影響が少なかった.呼吸性移動を加味した線量計算はS法が有用と考えられた. 続きを見る
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論文
Numazawa, Satomi ; Matsuzaka, Masashi ; Iwane, Kaori ; Inoue, Ryo ; Danjo, Kazuma ; Takahashi, Ippei ; Umeda, Takashi ; Nakaji, Shigeyuki
出版情報: 弘前医学.  61  pp.131-137,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4350
概要: The present study investigated the relationship between obesity and atherosclerosis according to agein the general femal e population. Six hundred fifty-five females were divided into 3 different age groups and therelationship between brachial-ankle pulse wave velocity (baPWV) and various obesity indices were investigated.In the youngest age group (20-39 years), BMI, percentage body fat and abdominal circumference were positivelycorrelated with baPWV. In the middle-aged group (40-59 years), all four obesity indices (BMI, percentage bodyfat, abdominal circumference and the waist-to-hip ratio (WHR)) were positively correlated with baPWV. However,baPWV was found to be negatively correlated with BMI only in the oldest age group (60 years and over), thoughit was still positively correlated with WHR (but not abdominal circumference). The reason for this may be due to agradual reduction of body weigh caused by some lifestyle-related diseases or aging. In this study, it was confirmedthat abdominal obesity is a key indicator of arteriosclerosis, though the importance of BMI (general obesity) as anindicator may depend on the generation. Furthermore, our results suggested that abdominal circumference and WHRmay have different significance as the indicators of arteriosclerosis. 続きを見る
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論文
Yamai, Kiyonori ; Umeda, Takashi ; Matsuzaka, Masashi ; Danjo, Kazuma ; Takahashi, Ippei ; Tsuya, Ryosuke ; Hasebe, Tatsuya ; Nakaji, Shigeyuki
出版情報: 弘前医学.  61  pp.138-149,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4351
概要: We examined the effects of vitamin C supplementation on neutrophil function during exercise loading.Neutrophil functions , namely the reactive oxygen species (ROS) production capability, neutrophil phagocytic activity(PA) and serum opsonic activity (SOA) were measured before and after a 2-hour unified loading exercise (ULE)both before and after a 7-day intensified training camp for 22 male judoists. The parameters were assessed neutrophilcount, myogenic enzymes, vitamin C in serum, SOA, PA, ROS production capability, body composition, and so on.Subjects were randomly assigned to two groups; the VC group (daily diet supplementation of 1,500 mg vitamin C)and the Control group (no vitamin C supplementation). The post-camp pre-ULE vitamin C level was higher in theVC group than in the Control group, though no such difference was seen at pre-camp. As for neutrophil function,although the typical changes seen following a single bout of normal exercise, namely an increase in SOA and ROS,and a decrease in PA, were recorded following the pre- and post-camp ULEs in both groups, significant differencein change rates were not seen between both groups. In conclusion, vitamin C supplementation had no significantinfluence on changes in neutrophil function. 続きを見る
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論文
Maruyama, Masateru ; Odagiri, Hiroki ; Ikenaga, Shojiro-Kazunori ; Nodagashira, Tatsuya ; Sato, Toshiyuki ; Hakamada, Kenichi ; Munakata, Hirofumi
出版情報: 弘前医学.  61  pp.150-158,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4352
概要: We examined the therapeutic efficacy for the suicide gene introduction using two recombinant adenovirusvectors with HER2 promoter and Cre/loxP system in human gastric cancer cell lines, MKN-7 and MKN-28. HER2protein level was more expressed in MKN-7 than in MKN-28. Next, we constructed a Cre recombinae expressionvector in HER2-producing cell specifically, AxHER2NCre and AxCALNCD expressing cytosine deaminase (CD)gene under the control of the CAG promoter by the Cre switching system. Much higher CD messenger RNA (CDmRNA) and CD protein expression were induced in cells by the double infection method than by AxCALNCD only.Furthermore, CD mRNA and CD protein expression were induced higher in HER2-overexpressing cell line, MKN-7 than in MKN-28. We examined the efficacy of cell growth inhibition using 5-fluorocytosine (5-FC) as anti-tumorprodrug. Inhibition effect was dose-dependent at each cell line and rate was more in MKN-7 in comparison withMKN-28. This system can be applied for HER2-overexpressing cancer specific gene therapy. 続きを見る
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論文
Kawaguchi, Yoko ; Hashiba, Eiji ; Kitayama, Masatou ; Hirota, Kazuyoshi
出版情報: 弘前医学.  61  pp.159-162,  2011-01-17.  弘前大学大学院医学研究科・弘前医学会
URL: http://hdl.handle.net/10129/4353
概要: A 30-year-old woman underwent an emergency cesarean section because of fetal distress involveduterine rupture. As the pl acenta was firmly adhered to the uterine wall, following safe delivery, it took over one hour and more than 10000 ml blood loss until the placenta was removed. Additional blood was ordered, but there was delay in delivery. Bleeding was extremely beyond our estimation, and hardly controlled despite of transfusing1400 ml of prepared blood. With stopping operation and continuing hyperoxic ventilation, we decided to start intraoperative blood salvage. Though hemoglobin, platelet, and base excess decreased to 2.7 g/dl, 1000/μl, and -11.2,hemodynamics could be maintained with use of a blood salvage system. Salvaged blood was very helpful to manage critical hemodilution until additional blood was reached. Overall estimated blood loss was 20190 ml and massive blood transfusion was needed. The patient emerged from anesthesia with no neurological complication. Rarely but actually cesarean section resulted in life-threatening hemorrhage. Blood salvage systems is safe and useful during cesareansection. Blood salvage should be usually considered one of the suitable choices in cesarean section patients when supply of homologous blood is limited. 続きを見る
9.

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論文
木村, 一之 ; 松崎, 康司 ; 北村, 英夫 ; 赤坂, 英二郎 ; 六戸, 大樹 ; 中野, 創 ; 今泉, 忠淳 ; 佐藤, 敬 ; 澤村, 大輔
出版情報: 弘前医学.  62  pp.1-6,  2011-03-15.  弘前大学出版会
URL: http://hdl.handle.net/10129/4417
概要: Retinoic acid-inducible gene-I(RIG-I)はウイルスの二本鎖RNAを認識しtype I interferon(IFN)の産生を誘導する細胞質蛋白である.ヒト表皮細胞ではRIG-IはIFN-γ およびtumor necrosis factor-α刺激によって誘導される.今回我々は,表皮への代表的な外的刺激であるウイルス感染と紫外線がヒト表皮細胞でのRIG-I発現に与える影響について検討した.擬似的ウイルス感染を生じるpolyinosinic-polycytidylic acidを用いてヒト表皮細胞を刺激するとRIG-Iの発現増強が認められたが,この発現増強と非刺激時のRIG-I基礎発現量は中波長紫外線(UVB)照射によって抑制された.このことよりUVB がRIG-I発現抑制を介して皮膚免疫の低下を促し,皮膚におけるウイルス感染やその再活性化の誘導に関与している可能性が示唆された. 続きを見る
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論文
西村, 美八 ; 檀上, 和真 ; 松坂, 方士 ; 津谷, 亮佑 ; 倉内, 静香 ; 古川, 照美 ; 高橋, 一平 ; 梅田, 孝 ; 兼板, 佳孝 ; 大井田, 隆 ; 中路, 重之
出版情報: 弘前医学.  62  pp.34-43,  2011-03-15.  弘前大学出版会
URL: http://hdl.handle.net/10129/4421
概要: 睡眠は生活の質(QOL)における重要な因子であり,包括的な睡眠状況の把握とその改善に向けた対策が求められている.本研究では一般住民を対象とし,睡眠障害と生活習慣の関連について検討した.平成19~21年度岩木健康増進プロジェクトに参加した1, 273名を対象とした.自記式アンケートにて生活習慣の聞き取りを行い,ピッツバーグ睡眠質問票(PSQI)により睡眠障害を判定した.対象者を男女別に分類し,年代を若年群,中年群,高齢群に区分した.PSQIの得点を従属変数とし,BMI,喫煙習慣,飲酒習慣,抑うつの有無を独立変数とした重回帰分析を行った.その結果,男女とも,すべての年代において,睡眠障害とうつとの間に正の相関を示した.以上より,睡眠障害の予防と良好な睡眠習慣の獲得のためには精神的なサポートが重要であり,これによりQOLの改善が可能であることが示唆された. 続きを見る