Upregulation of Insulin-Like Growth Factor-1 Gene Expression in Cardiac Myocytes by Hypoxia : Mechanism for Its Cardioprotective Effect

フォーマット:

論文

責任表示:

Kato, Chisato ; Osanai, Tomohiro ; Shibutani, Shuji ; Hanada, Kenji ; Okumura, Ken

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2009-03-31

著者名:

• Kato, Chisato
• Osanai, Tomohiro
• Shibutani, Shuji
• Hanada, Kenji
• Okumura, Ken

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

60

通号:

1/2/3/4

開始ページ:

36

終了ページ:

44

バージョン:

publisher

概要:

Insulin-like growth factor （IGF）-1 is known to exert benefi cial eff ects on the heart, but its source andfunction under hypoxia are unknown. We investigated the eff ect of hypoxia on IGF-1 expression and its role in theregeneration in the … heart. Cardiac myocytes and fi broblasts obtained from neonate mice heart were cultured andexposed to hypoxia. mRNA of IGF-1, IGF-binding protein 3 （IGFBP3）, and vascular endothelial growth factor-A（VEGF-A） was measured by real-time PCR. In cardiac myocytes, IGF-1 mRNA was increased by 3.5±1.1 fold at 3hours after hypoxia concomitantly with the increase in IGFBP3 and VEGF-A mRNA, and returned to the baseline at24 hours. In contrast, IGF-1 mRNA in cardiac fi broblasts was unchanged by hypoxia, although VEGF-A mRNA wasincreased. To investigate the role of IGF-1 in the heart regeneration, we measured the gene expressions of stromalcell-derived factor-1（ SDF-1）, its receptor CXCR4, and matrix metalloproteinase（ MMP）-14 related to cell homing. Incardiac myocytes, SDF-1 and MMP-14 mRNA were increased at 3 hours after hypoxia and tended to be positivelycorrelated with IGF-1 mRNA. These suggest that hypoxia increases IGF-1 expression in cardiac myocytes, and thisendogenous IGF-1 may exert benefi cial eff ects on regeneration in the heart. 続きを見る

URL:

http://hdl.handle.net/10129/1796