Vimentin intermediate filament and plectin provide a scaffold for invadopodia, facilitating cancer cell invasion and extravasation for metastasis

フォーマット:

学位論文

責任表示:

Yoneyama, Mihoko

言語:

英語

出版情報:

Elsevier, 2014-04

著者名:

Yoneyama, Mihoko  

掲載情報:

European Journal of Cell Biology

ISSN:

0171-9335      

巻:

93

通号:

4

開始ページ:

157

終了ページ:

169

バージョン:

ETD

概要:

To investigate the molecular mechanisms of cancer metastasis, we have isolated a high-metastatic bladder cancer cell subpopulation from a low-metastatic cell line by using an in vivo selection system. Cells in the subpopulation showed a hig … h ability to form invadopodia, the filamentous actin (F-actin)-based membrane protrusions that play an essential role in cancer cell invasion. Analysis of the gene expression profile revealed that the expression of an intermediate filament (IF) protein, vimentin and a cytoskeletal linker protein, plectin was up-regulated in the high-metastatic subpopulation compared with the low metastatic cell line. Here we report a novel role of vimentin IF and plectin in metastasis. In invasive bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link was formed. Disruption of this link severely impaired invadopodia formation, reducing the capacities of extracellular matrix degradation, transendothelial migration and metastasis. In addition, the vimentin assembly into the filaments was required for invadopodia formation. Our results suggest that plectin anchoring invadopodia to vimentin IF scaffolds and stabilizes invadopodia, which is a critical molecular process for cancer cell invasion and extravasation for metastasis.<br />掲載誌本文リンク：http://www.sciencedirect.com/science/article/pii/S017193351400048X 続きを見る

URL:

http://hdl.handle.net/10129/5477