Innate Immune Reactions Against RNA Viruses in Renal Mesangial Cells

Innate Immune Reactions Against RNA Viruses in Renal Mesangial Cells

フォーマット:

論文

責任表示:

Imaizumi, Tadaatsu ; Aizawa, Tomomi ; Tanaka, Hiroshi ; Sato, Fuyuki ; Xing, Fei ; Matsumiya, Tomoh ; Yoshida, Hidemi ; Satoh, Kei

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2013-04-02

著者名:

掲載情報:

弘前医学

ISSN:

0439-1721

巻:

64

通号:

Supplement

開始ページ:

S70

終了ページ:

S73

バージョン:

publisher

概要:

　Viral infection is important in renal pathology both as a trigger of chronic inflammatory diseases and as a complication associated with organ transplantation. Glomerular mesangial cells produce a variety of functional molecules potentiall … y involved in immune reactions, and we investigated anti-viral responses in normal human mesangial cells. Human mesangial cells were treated with polyinosinic-polycytidylic acid （poly IC）, an authentic double-stranded RNA that mimics viral RNA. Treatment of cells with poly IC induced interferon-β （IFN-β）, retinoic acid-inducible gene-I（ RIG-I）, CC chemokine ligand 5（ CCL5）, differentiated embryo-chondrocyte 2（ DEC2） and IFN-stimulated gene 20 （ISG20）. Knockdown of toll-like receptor 3 （TLR3）, by RNA interference （RNAi）, abolished the poly IC-induced expression of these molecules. RNAi against IFN-β inhibited the induction of RIG-I,CCL5 and ISG20, but not of DEC2. Knockdown of RIG-I resulted in the reduced expression of CCL5. RNAi against DEC2 enhanced the poly IC-induced expression of IFN-β, RIG-I and CCL5. Transfection of cells with a poly IC/cationic lipid complex induced IFN-β, RIG-I and ISG20. Knockdown of RIG-I decreased the expression of IFN-β and ISG20 induced by transfection with poly IC/cationic lipid. TLR3 and RIG-I may function as recognition receptors against double-stranded RNA, which induce IFN-β and its downstream IFN-inducible genes. In the signaling elicited by poly IC, the IFN-inducible genes include RIG-I and effector molecules as CCL5 with leukocyte chemotactic activity and ISG20 with exonuclease activity on single-stranded RNA. The poly IC-induced expression of DEC2 is independent on IFN-β and it may control the signaling elicited by double-stranded RNA. The poly IC-inducible molecules may mediate anti-viral innate responses in renal mesangial cells. 続きを見る

URL:

http://hdl.handle.net/10129/4869

タイトルが類似している資料

詳細

主題:	DEC2; FN-β; ISG20; RIG-I; TLR3; mesangial cells
受理日:	2013-04-02

類似資料:

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2 論文 Cylindromatosis (CYLD), a Deubiquitinase, Attenuates Inflammatory Signaling Pathways by Activating Toll-Like Receptor 3 in Human Mesangial Cells. Imaizumi, Tadaatsu, Hayakari, Ryo, Watanabe, Shojiro, Aizawa, Tomomi, Matsumiya, Tomoh, Yoshida, Hidemi, Tsuruga, …	8 論文 Clock Gene Expressions are Altered by Co-culture of Gingival Fibroblasts and Oral Cancer Cells Furudate, Ken, Matsumiya, Tomoh, Hayakari, Ryo, Xing, Fei, Kubota, Kosei, Sakaki, Hirotaka, Tamura, Yoshihiro, Kijima, … 弘前大学大学院医学研究科・弘前医学会
3 論文 Urinary fractalkine and monocyte chemoattractant protein-1 as possible predictors of disease activity of childhood glomerulonephritis. Aizawa, Tomomi, Imaizumi, Tadaatsu, Tsuruga, Kazushi, Watanabe, Shojiro, Yoshida, Hidemi, Kumagai, Naonori, Ito, Etsuro, …	9 論文 Effect of low-concentration amyloid-β 1–42 (Aβ42) on human neuroblastoma SH-SY5Y cell viability: neuroprotective potential of combination use with carnosic … Yoshida, Hidemi, Hashimoto, Yuko, Fukushima, Takashi, Tanji, Kunikazu, Matsumiya, Tomoh, Seya, Kazuhiko, Kawaguchi, … 弘前大学大学院医学研究科・弘前医学会
4 論文 Interferon (IFN)-Induced Protein 35 (IFI35), a Type I Interferon-Dependent Transcript, Upregulates Inflammatory Signaling Pathways by Activating Toll-Like … Imaizumi, Tadaatsu, Yano, Chikashi, Numata, Akiko, Tsugawa, Koji, Hayakari, Ryo, Matsumiya, Tomoh, Yoshida, Hidemi, …	10 論文＜Symposium I＞Vascular endothelial growth factor（ VEGF） expression is negatively regulated by basic-helix-loop-helix（ bHLH）transcription factor DEC2 Kijima, Hiroshi, Sato, Fuyuki, Bhawal, Ujjal Kumar, Kawamoto, Takeshi, Fujimoto, Katsumi, Imaizumi, Tadaatsu, Imanaka, … 弘前大学大学院医学研究科・弘前医学会
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