Expression and Function of the P2y14 Receptor in Murine Peritoneal Macrophages

フォーマット:

論文

責任表示:

Kudo, Fujimi ; Nishiguchi, Naoki ; Ito, Kyoko ; Nakano, Manabu ; Ito, Koichi

言語:

日本語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2012-11-30

著者名:

• Kudo, Fujimi
• Nishiguchi, Naoki
• Ito, Kyoko
• Nakano, Manabu
• Ito, Koichi

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

63

通号:

2-4

開始ページ:

96

終了ページ:

104

バージョン:

publisher

概要:

The P2Y14 receptor is activated by UDP-glucose （UDPG）, which is a well-known glycosyl donor that participates in the biosynthesis of carbohydrates, and is widely expressed in immune cells. During inflammation and mechanical stress, damaged … cells release nucleotides, including ATP and UDPG, as danger signals that act as P2Y receptor agonists. These nucleotide-induced signals participate in the regulation of immune responses. In this study, to investigate P2Y14 expression further, we performed flow cytometric analysis using an anti-P2Y14 monoclonal antibody. The results indicated that P2Y14 is expressed in murine immune cells, including T cells, B cells, monocytes, granulocytes, and CD11bhigh macrophages. Interestingly, the expression levels of P2Y14 differed between immature and mature monocytes, and in CD11bhigh macrophages, P2Y14 was gradually downregulated as peritonitis was terminated. The expression of CD11b is reduced by the efferocytosis of apoptotic neutrophils during peritonitis, and the induced CD11blow macrophages, which emerge in the resolution of peritonitis, play an important role in the termination of inflammation. Consistent with this observation, we revealed that administration of UDPG to mice with induced peritonitis increased the number of CD11blow macrophages. As P2Y14 is expressed in CD11bhigh macrophages but not in CD11blow macrophages, UDPG may participate in the conversion to the CD11blow phenotype. These data suggest a novel regulatory pathway of the inflammatory response via P2Y14 expressed on macrophages. 続きを見る

URL:

http://hdl.handle.net/10129/4811