THE SUICIDE GENE INTRODUCTION USING TWO RECOMBINANT ADENOVIRUS VECTORS WITH HER2 PROMOTER AND Cre/loxP SYSTEM

フォーマット:

論文

責任表示:

Maruyama, Masateru ; Odagiri, Hiroki ; Ikenaga, Shojiro-Kazunori ; Nodagashira, Tatsuya ; Sato, Toshiyuki ; Hakamada, Kenichi ; Munakata, Hirofumi

言語:

日本語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2011-01-17

著者名:

Maruyama, Masateru

Odagiri, Hiroki

Ikenaga, Shojiro-Kazunori

Nodagashira, Tatsuya

Sato, Toshiyuki

Hakamada, Kenichi

Munakata, Hirofumi

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掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

61

通号:

2-4

開始ページ:

150

終了ページ:

158

バージョン:

publisher

概要:

We examined the therapeutic efficacy for the suicide gene introduction using two recombinant adenovirusvectors with HER2 promoter and Cre/loxP system in human gastric cancer cell lines, MKN-7 and MKN-28. HER2protein level was more expressed … in MKN-7 than in MKN-28. Next, we constructed a Cre recombinae expressionvector in HER2-producing cell specifically, AxHER2NCre and AxCALNCD expressing cytosine deaminase （CD）gene under the control of the CAG promoter by the Cre switching system. Much higher CD messenger RNA （CDmRNA） and CD protein expression were induced in cells by the double infection method than by AxCALNCD only.Furthermore, CD mRNA and CD protein expression were induced higher in HER2-overexpressing cell line, MKN-7 than in MKN-28. We examined the efficacy of cell growth inhibition using 5-fluorocytosine （5-FC） as anti-tumorprodrug. Inhibition effect was dose-dependent at each cell line and rate was more in MKN-7 in comparison withMKN-28. This system can be applied for HER2-overexpressing cancer specific gene therapy. 続きを見る

URL:

http://hdl.handle.net/10129/4352