ANALYSIS OF FUNCTIONAL SUGAR CHAINS OF PROTEOGLYCANS IN THE URINE FROM WOMEN WITH OSTEOPOROSIS

フォーマット:

論文

責任表示:

Abe, Kazuhiro ; Higuchi, Tsuyoshi ; Mizunuma, Hideki ; Kakizaki, Ikuko ; Endo, Masahiko ; Suto, Shinichiro

言語:

日本語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2011-01-17

著者名:

Abe, Kazuhiro

Higuchi, Tsuyoshi

Mizunuma, Hideki

Kakizaki, Ikuko

Endo, Masahiko

Suto, Shinichiro

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掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

61

通号:

2-4

開始ページ:

97

終了ページ:

103

バージョン:

publisher

概要:

The aim of study is to analyze functional sugar chains, glycosaminoglycans （GAGs） in urine frompostmenopausal women with osteoporosis. A 24-hour urine was collected from 15 osteoporotic women and normalwomen. All women were postmenopausal a … nd aged at 60 to 70 years old. Diagnosis of osteoporosis was based onthe diagnosis criteria proposed by the Japan Osteoporosis Society after measurement of lumbar spine bone mineraldensity by dual energy X-ray absorptiometry. The urine was concentrated by dialysis and evaporation, thenprecipitated with ethanol. The precipitate was then reconstituted into NaCl solution, precipitated again by adding 10% cetylpyridinium chloride and the precipitation was used as complex GAGs fraction. The yield of complex fractionwas significantly higher in osteoporotic subjects than that in normal control. Then, the complex GAGs fraction wasanalyzed by cellulose acetate membrane electrophoresis and high performance liquid chromatography （HPLC）,respectively. Cellulose acetate membrane electrophoresis has revealed that GAGs in urine contained partiallydesulfated chondroitin sulfate. However, further analysis by HPLC has shown that the excretion of desulfated GAGswas rather less in osteoporotic women, indicating that urinary GAGs fraction from osteoporotic women containsunknown acidic polysaccharides as well as GAGs. 続きを見る

URL:

http://hdl.handle.net/10129/4346