＜Poster＞Neurovascular coupling studies in awake-behaving mice

フォーマット:

論文

責任表示:

Takuwa, Hiroyuki ; Masamoto, Kazuto ; Obata, Takayuki ; Kanno, Iwao

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2010-07-08

著者名:

• Takuwa, Hiroyuki
• Masamoto, Kazuto
• Obata, Takayuki
• Kanno, Iwao

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

61

通号:

Supplement

開始ページ:

S192

終了ページ:

S196

バージョン:

publisher

概要:

The mechanism regulating cerebral blood fl ow （CBF） during brain function （neurovascular coupling）has been widely investigated in animals under anesthetized conditions, though anesthesia is known to greatly aff ect neurovascular physiology. … The present study aims to develop a novel model for neurovascular coupling studies in awake-behaving mice. Male C57BL/6J mice were initially anesthetized with isofl urane in preparation for attaching the study apparatus to the head. The animal was tethered to the study apparatus but allowed to move spontaneously on a floated ball. The animal behavior and regional CBF in the somatosensory barrel cortex were simultaneously measured with optical motion sensor and laser-Doppler fl owmetry （LDF）, respectively. Anesthesia was discontinued during recovery, while whisker stimulation （frequency 10 Hz and duration 10 or 20 sec） was induced at either the contralateral or ipsilateral side of the LDF recording site. During the experiments, the animals showed no signs of struggling against the head restraint. The intensity of baseline CBF was higher while the animal was under 2% isoflurane aesthesia than it was after anesthesia was stopped. CBF response to stimulation was not observed under anesthesia. After the animal was recovered from anesthesia, an increase in CBF （34 ± 18%） was observed during contralateral stimulation but not during ipsilateral stimulation. The fl uctuation levels of CBF baseline during resting and walking conditions were ±2.7% and ±3.5%, respectively.　We observed that these fl uctuations were not due to vibration noises caused by such as air-puff and animal motion in our experimental conditions. 続きを見る

URL:

http://hdl.handle.net/10129/3687