Reconstitution of the Immune System after Murine Allogeneic Umbilical Cord Blood Transplantation

フォーマット:

論文

責任表示:

Sato, Hideaki ; Sato, Masashi ; Chiba, Makie ; Ito, Kyoko ; Ito, Koichi

言語:

日本語

出版情報:

弘前大学出版会, 2010-03-25

著者名:

• Sato, Hideaki
• Sato, Masashi
• Chiba, Makie
• Ito, Kyoko
• Ito, Koichi

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

61

通号:

1

開始ページ:

35

終了ページ:

45

バージョン:

publisher

概要:

The ability of murine allogeneic umbilical cord blood cells （UCBCs） to reconstitute the immune systemwas investigated. UCBCs obtained from fetuses of C57BL/6 （B6; H-2b） mice, which were transgenic for greenfluorescent protein （GFP）, were tr … ansplanted into RAG2 （-/-） BALB/c mice （H-2d）. After transplantation, flowcytometric analysis revealed successful reconstitution of phenotypically mature GFP-positive immune cells of donororigin, including T cells, B cells, monocytes, and granulocytes in the peripheral blood of the recipient mice. Analysisof functional maturation of lymphocytes revealed that 2,4,6-trinitrophenyl-keyhole limpet hemocyanin （TNP-KLH）-immunized UCBC-transplanted recipients produced both TNP-specific IgM and IgG antibodies. These resultsindicated that the recipient mice were capable of mounting antibody responses to T-dependent antigens; further, Igclass switching from IgM to IgG confi rmed that both B cells and CD4+ helper T cells derived from allogeneic UCBCswere immunologically competent. Furthermore, mice transplanted with allogeneic UCBCs accepted skin grafts fromboth B6 and BALB/c mice. However, these chimeric mice completely rejected skin grafts from third party C3H/HeJ （H-2k） mice, indicating the presence of functional CD8+ killer T cells as well as CD4+ helper T cells. In termsof potential clinical application, our results indicate that allogeneic UCBC transplantation can enable recovery of thenormal immune system in recipients. 続きを見る

URL:

http://hdl.handle.net/10129/3278