Salmon cartilage proteoglycan modulates cytokine responses to Escherichia coli in mouse macrophages

フォーマット:

論文

責任表示:

Sashinami, Hiroshi ; Takagaki, Keiichi ; Nakane, Akio

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2007-11-29

著者名:

• Sashinami, Hiroshi
• Takagaki, Keiichi
• Nakane, Akio

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

59

通号:

Supplement

開始ページ:

S235

終了ページ:

S243

バージョン:

publisher

概要:

Proteoglycans （PGs） are complex glycohydrates, which are widely distributed in connective tissues andon the cell surface of mammalian tissues. We investigated the effect of PG extracted from salmon cartilage oncytokine responses to stimulat … ion with heat-killed Escherichia coli（ HKEC） in a mouse macrophage cell line, RAW264.7.PG exhibited the suppression of tumor necrosis factor（ TNF）-α production and enhancement of interleukin（ IL）-10production compared with chondroitin 4 sulfate（ C4S） and chondroitin 6 sulfate（ C6S）. PG, C4S and C6S suppressedHKEC-induced Toll-like receptor 4（ TLR4） and inducible nitric oxide synthase（ iNOS） expression dose-dependentlyand the PG showed the strongest suppressive effect among 3 compounds. Only PG dramatically up-regulated theexpression of signal transducers and activators of transcription 3（ STAT3） and the phosphorylation of STAT3 in mousemacrophages. Our results showed strong suppression of PG on infl ammatory response and suggested that the novelinteraction might exist between the extracellular matrix and immune system. 続きを見る

URL:

http://hdl.handle.net/10129/2241