Leptin can suppress the transcription of corticotropin-releasing factor in the immortalized hypothalamic neuron

フォーマット:

論文

責任表示:

Sakihara, Satoru ; Kageyama, Kazunori ; Nigawara, Takeshi ; Hanada, Komaki ; Suda, Toshihiro

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2007-11-29

著者名:

• Sakihara, Satoru
• Kageyama, Kazunori
• Nigawara, Takeshi
• Hanada, Komaki
• Suda, Toshihiro

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

59

通号:

Supplement

開始ページ:

S193

終了ページ:

S198

バージョン:

publisher

概要:

Corticotropin-releasing factor （CRF） is primary regulator of hypothalamus-pituitary-adrenal（ HPA） axis,which is activated by various types of stress. Starvation is also a potent activating factor of CRF. Because theperipheral energy store r … efl ects on plasma levels of leptin, secreted from adipose tissue, it is presumed that leptin maybe involved in the CRF activity during starvation. Indeed, the attenuation of CRF or HPA axis activity by leptintreatment has been demonstrated in several reports. While many studies indicate the leptins involvement in CRFactivity, its precise regulation has not been elucidated. The aim of this study is to determine the impact of leptin onthe hypothalamic CRF neuron. To exclude the other hypothalamic factors, associated with leptins central eff ect, weemployed the in vitro study using immortalized CRF expressing neuron（ IVB cell）. Since the mRNA expression of longformleptin receptor was detected clearly in IVB cell with RT-PCR, we next investigated the impact of leptin on this cell.IVB cell was treated with leptin in diff erent concentrations and periods, then, the mRNA expression and the promoteractivity of CRF were analyzed with real-time PCR and luciferase assay, respectively. In result, leptin suppressed CRFpromoter activity with dose- and time- dependent manner in IVB cell. This indicates that leptin can suppress the CRFtranscription directly in hypothalamus. Our result might explain the mechanism partially how leptin attenuate theactivation of CRF or HPA axis induced by stress or starvation. 続きを見る

URL:

http://hdl.handle.net/10129/2235