The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro

フォーマット:

論文

責任表示:

Kusumi, Akinori ; Sakaki, Hirotaka ; Kusumi, Tomomi ; Nakagawa, Hiroshi ; Kubota, Kosei ; Oda, Mitsuo ; Satoh, Hisashi ; Kimura, Hiroto

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2007-11-29

著者名:

Kusumi, Akinori

Sakaki, Hirotaka

Kusumi, Tomomi

Nakagawa, Hiroshi

Kubota, Kosei

Oda, Mitsuo

Satoh, Hisashi

Kimura, Hiroto

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掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

59

通号:

Supplement

開始ページ:

S172

終了ページ:

S187

バージョン:

publisher

概要:

The application of mechanical stress is known to be the unique method of analyses on bone metabolismand remodeling in vitro and in vivo. We have investigated on the effects of the application of cyclic tensile strain（CTS） on the syntheses o … f bone metabolic factors from the normal human osteoblasts in vitro. In this study, we foundthat diff erent passage numbers aff ected synthesis of CTS-induced factors from osteoblasts in vitro. Osteoblasts werestimulated with the application of CTS for 3 days, 4 hrs a day. Then, it was found that the application of CTS increasednitric oxide（ NO） production, the expression of cyclooxygenase 2（ Cox-2） mRNA, and synthesis of osteopontin（ OPN）from osteoblasts during serial passage. However, CTS-induced osteoprotegerin（ OPG） synthesis from osteoblasts wasshown to change at diff erent passage numbers. In short, increasing at the 3rd passage, CTS-induced OPG synthesis fromosteoblasts decreased at the 5th passage. While, though soluble RANKL（ sRANKL） release by the application of CTSdecreased at 1st passage and did not change at the 5th passage, the expression of receptor activator of nuclear factor-κBligand（ RANKL） mRNA increased in osteoblasts at the 5th passage, decreasing in those at the 3rd passage. The increaseon OPN synthesis the expression of Cox-2 mRNA, and NO production from osteoblasts by the application of CTS wasnot aff ected during serial passage. Analyzing on the activity of the p38 mitogen-activated protein kinase（ p38 MAPK）in osteoblasts with or without the application of CTS, the p38 MAPK activity in osteoblasts at the 3rd passage inhibitedin compared with that at the 3rd passage. From these results, we should use osteoblasts at the 1st passage whenanalyzing on CTS-induced OPG synthesis with normal human osteoblasts. It is suggested that the regulation of CTSinducedOPG synthesis might be aff ected by aging of osteoblasts. 続きを見る

URL:

http://hdl.handle.net/10129/2233