The role of NEDD8 Ultimate Buster 1, NUB1, in neurodegenerative α-synucleinopathies
- フォーマット:
- 論文
- 責任表示:
- Tanji, Kunikazu ; Mori, Fumiaki ; Takahashi, Hitoshi ; Kamitani, Tetsu ; Wakabayashi, Koichi
- 言語:
- 英語
- 出版情報:
- 弘前大学大学院医学研究科・弘前医学会, 2007-11-29
- 著者名:
- 掲載情報:
- 弘前医学
- ISSN:
- 0439-1721
- 巻:
- 59
- 通号:
- Supplement
- 開始ページ:
- S143
- 終了ページ:
- S146
- バージョン:
- publisher
- 概要:
- NEDD8 is developmentally down-regulated ubiquitin-like protein, which can conjugate covalently to their target proteins. Recently, we identifi ed NUB1 as a NEDD8-interacting protein, which is composed of 601 amino acid residues with a calculated molecular mass of 69.1 kDa. More recently, we showed that NUB1 is an interferon-induced protein and also recruits NEDD8 to the proteasome for degradation. Here, we performed a yeast two-hybrid screening using NUB1 as bait … and isolated the cDNA of synphilin-1 from a human testis cDNA library. Synphilin-1 is a major component of inclusion bodies found in the brains of patients with α-synucleinopathies, including Parkinson’s disease. Our biochemical study showed that NUB1 directly interacts with synphilin-1 through its NEDD8-binding site. In addition, our immunohistochemical study showed that NUB1, as well as synphilin-1, accumulates in the inclusion bodies found in the brains of patients with α-synucleinopathies. These fi ndings imply that NUB1 plays a role in the formation of synphilin-1-positive inclusions. 続きを見る
- URL:
- http://hdl.handle.net/10129/2228
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