Upregulation of Insulin-Like Growth Factor-1 Gene Expression in Cardiac Myocytes by Hypoxia : Mechanism for Its Cardioprotective Effect
- フォーマット:
- 論文
- 責任表示:
- Kato, Chisato ; Osanai, Tomohiro ; Shibutani, Shuji ; Hanada, Kenji ; Okumura, Ken
- 言語:
- 英語
- 出版情報:
- 弘前大学大学院医学研究科・弘前医学会, 2009-03-31
- 著者名:
- 掲載情報:
- 弘前医学
- ISSN:
- 0439-1721
- 巻:
- 60
- 通号:
- 1/2/3/4
- 開始ページ:
- 36
- 終了ページ:
- 44
- バージョン:
- publisher
- 概要:
- Insulin-like growth factor (IGF)-1 is known to exert benefi cial eff ects on the heart, but its source andfunction under hypoxia are unknown. We investigated the eff ect of hypoxia on IGF-1 expression and its role in theregeneration in the … heart. Cardiac myocytes and fi broblasts obtained from neonate mice heart were cultured andexposed to hypoxia. mRNA of IGF-1, IGF-binding protein 3 (IGFBP3), and vascular endothelial growth factor-A(VEGF-A) was measured by real-time PCR. In cardiac myocytes, IGF-1 mRNA was increased by 3.5±1.1 fold at 3hours after hypoxia concomitantly with the increase in IGFBP3 and VEGF-A mRNA, and returned to the baseline at24 hours. In contrast, IGF-1 mRNA in cardiac fi broblasts was unchanged by hypoxia, although VEGF-A mRNA wasincreased. To investigate the role of IGF-1 in the heart regeneration, we measured the gene expressions of stromalcell-derived factor-1( SDF-1), its receptor CXCR4, and matrix metalloproteinase( MMP)-14 related to cell homing. Incardiac myocytes, SDF-1 and MMP-14 mRNA were increased at 3 hours after hypoxia and tended to be positivelycorrelated with IGF-1 mRNA. These suggest that hypoxia increases IGF-1 expression in cardiac myocytes, and thisendogenous IGF-1 may exert benefi cial eff ects on regeneration in the heart. 続きを見る
- URL:
- http://hdl.handle.net/10129/1796
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弘前大学大学院医学研究科・弘前医学会 |