A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells

フォーマット:

論文

責任表示:

Niwa, Hidetomo ; Sakai, Tetsuhiro ; Furukawa, Ken-Ichi ; Kanemaru, Kota ; Hirota, Kazuyoshi

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2009-03-31

著者名:

• Niwa, Hidetomo
• Sakai, Tetsuhiro
• Furukawa, Ken-Ichi
• Kanemaru, Kota
• Hirota, Kazuyoshi

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

60

通号:

1/2/3/4

開始ページ:

27

終了ページ:

35

バージョン:

publisher

概要:

In the present study, we have conducted cDNA microarray in C6, rat brain glioma cell line, to assess anti-infl ammatory eff ects of ketamine and sevofl urane in the central nervous system. The cultured C6 cells were treated with ketamine （0 … -100 μM） and sevofl urane （0 and 0.66 mM）. Total RNA was extracted from the cells and labeled with fl uorescent dye and then hybridized with microarray slide, containing 1936 genes. Quantitative analysis of each gene expression was confi rmed by real-time polymerase chain reaction （PCR）. Microarray analyses showed that ketamine downregulated the expression of 4 proinfl ammatory cytokine genes and upregulated that of 2 antiinfl ammatory cytokine genes. On the other hand, sevofl urane downregulated the expression of 2 proinfl ammatory cytokines but upregulated that of two other proinfl ammatory cytokines. Furthermore, sevofl urane failed to stimulate the expressions of anti-infl ammatory cytokines. Although patterns of cytokine expression in response to ketamine and sevofl urane were diff erent from each other described above, both anesthetics downregulated a key cytokine, interleukin（ IL）-1β remarkably in microarray analysis, which was confi rmed by real time PCR. These results suggest that both ketamine and sevofl urane show mainly anti-infl ammatory properties through the inhibition of IL-1β. 続きを見る

URL:

http://hdl.handle.net/10129/1795