Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts

フォーマット:

論文

責任表示:

Endo, Yasufumi ; Takahashi, Gen ; Kakizaki, Ikuko ; Endo, Masahiko

言語:

英語

出版情報:

弘前大学大学院医学研究科・弘前医学会, 2019-11-01

著者名:

• Endo, Yasufumi
• Takahashi, Gen
• Kakizaki, Ikuko
• Endo, Masahiko

掲載情報:

弘前医学

ISSN:

0439-1721      

巻:

70

通号:

1

開始ページ:

1

終了ページ:

12

バージョン:

publisher

概要:

Abstract: Hyaluronan （HA） is a ubiquitous glycosaminoglycan comprising N-acetylglucosamine and glucuronic acid and is involved in matrix formation and cell maintenance. 4-Methylumbelliferone （MU） exhibits anti-tumor activities by inhibiting … HA synthesis. In this study, we aimed to elucidate the association between HA and numerous matrix molecules.Materials and Methods: We created HA-knock down extracellular matrix （ECM） by culturing human skin fibroblasts（ HSF） in the presence of MU（ 0.5 mM） for 3 days after confluence. Following culture in the presence or absence of MU, the cell layer and culture medium were collected and ECM molecules were measured using enzymelinked immunosolvent assay, and the［ 35S］-sulfate uptake method.Results: Proteoglycans and type I collagen were released into the medium as HA decreased. However, type collagen, but not proteoglycans, was immediately replenished. Fibronectin and CD44 in the cell layer decreased, but were not replenished, as HA decreased. On contrary, laminin, cadherin, and vitronectin were not affected by HA decrease.Conclusions: HA knockdown by MU treatment elucidated the dynamic interactions between HA and ECM components. Our findings suggest that HA interacts with other ECM components through various ways, including 続きを見る

URL:

http://hdl.handle.net/10129/00006844