Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia
- フォーマット:
- 論文
- 責任表示:
- Kimura, Toshiro ; Imaizumi, Tadaatsu ; Yoshida, Tatsuya ; Shimada, Taku ; Hayakari, Ryo ; Kawaguchi, Shogo ; Yoshida, Hidemi ; Kobayashi, Tamotsu ; Hirabayashi, Takeshi ; Mizukami, Hiroki ; Kijima, Hiroshi ; Hakamada, Kenichi
- 言語:
- 英語
- 出版情報:
- 弘前大学大学院医学研究科・弘前医学会, 2019-03-15
- 著者名:
- 掲載情報:
- 弘前医学
- ISSN:
- 0439-1721
- 巻:
- 69
- 通号:
- 1-4
- 開始ページ:
- 86
- 終了ページ:
- 94
- バージョン:
- publisher
- 概要:
- Purpose: Retinoic acid-inducible gene-I （RIG-I） is a member of cytoplasmic viral sensors which plays an important role in inflammation of biliary epithelial cells（ BECs）. The aim of this study is to examine if RIG-I and C-X-C motif chemokin … e 10（ CXCL10） are involved in the etiology of human biliary atresia（ BA）.Methods: Immunohistochemical study was performed on surgically resected tissues obtained（ June 1994 to March 2016）from 30 infants with BA and non-inflamed hepatic tissues from 7 infants with hepatoblastoma. A semiquantitative scoring system was designed to evaluate the staining with an antibodies to the RIG-I and CXCL10. The expression of RIG-I and CXCL10 in HuCCT1 cholangiocarcinoma cell line were studied by western blotting, ELISA and RT-PCR analyses.Results: Intense immunoreactivity for RIG-I and CXCL10 was detected in BECs in tissues resected from BA patients.The expression of RIG-I and CXCL10 in the hilar tissue was significantly stronger than in the hepatic tissue.Transfection of HuCCT1 cells with poly（I:C）, a synthetic analog of viral dsRNA, induced the expression of RIG-I, and knockdown of RIG-I inhibited the induction of CXCL10 in HuCCT1 cells transfected with poly（I:C）.Conclusion: These results suggest that RIG-I-CXCL10 cascade may be involved in the etiology of human BA. 続きを見る
- URL:
- http://hdl.handle.net/10129/00006586
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弘前大学大学院医学研究科・弘前医学会 |
弘前大学大学院医学研究科・弘前医学会 |
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弘前大学大学院医学研究科・弘前医学会 | |
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弘前大学大学院医学研究科・弘前医学会 | |
